The Plant Transformation Facility (PTF) is a service facility of the College of Agriculture and Life Sciences (CALS) and its School of Integrative Plant Science (SIPS). PTF is charged with supporting the plant biology research of Cornell faculty by making transgenic and genome-edited plants of time-consuming or hard-to-transform species. With the explosion of interest in genome-editing technologies, most of our standard plant transformations are currently for performing CRISPR/Cas9-mediated genome modification. Current projects at PTF concentrate on rice, maize, wheat, apple, and industrial hemp. The selection of species of focus at PTF is made to complement rather than compete with the Boyce Thompson Institute s Biotechnology Center, which transforms solanaceous species, Brachypodium distachyon, and Setaria viridis among others.
This position is heavily involved in the entire process of plant transformation, as well as lab management and record keeping.
* This individual will take the lead in plant transformations and genome editing. Most of our work involves sterile culture, so the individual will be involved in the preparation of a wide variety of sterile growth media for plants, Agrobacterium, and E. coli, and handle the transfer of plant tissues between media. The method of plant transformation varies for each species and even for different genotypes, and the technician will be involved in the development of new methods as necessary. Most transformations use Agrobacterium tumefaciens for DNA delivery, but we also have a gene gun and would like to explore protoplast transformation. The technician will use CRISPR/Cas9 and/or other genome-editing methods to alter plant genomes. The individual will select successful transformants using antibiotics or herbicides, and validate the results by extracting DNA and performing PCR. Transgenic plants will be regenerated from resistant callus tissue. The technician will also handle the care and maintenance of plants grown in tissue culture, as well as in soil-less mixes in growth chambers and the greenhouse.
* In terms of lab management, the individual will maintain an inventory of all lab supplies, ensure that the lab does not run low on any supplies, place orders, and make purchasing recommendations as required; maintain the order and cleanliness of the lab, including cleaning and maintaining dishes and equipment; supervise and schedule the use of equipment and lab materials; ensure lab compliance with safety procedures, including appropriate labeling and disposal of laboratory chemical waste; maintain an online database of all established laboratory protocols; provide orientation and training for new members or visitors in the lab; supervise and advise any undergraduates or visitors in the lab; and recommend improvements to the safety, workflow, organization, efficiency, costs, and overall success of the lab.
* The technician will play a major role in record keeping for the lab. Careful maintenance and organization of DNA, Agrobacterium, seed stocks, and other materials for plant transformation will be important. This individual will keep detailed, accurate records, procedures, and results of experiments and data in a laboratory notebook; help establish, update, and maintain an online database of the status of all transgenic projects; help establish, update, and maintain a lab database of all plant materials in our possession, including individual transgenic lines; and provide occasional written and oral project status reports using Word, Excel, and/or PowerPoint.
THIS IS A FULL-TIME (39 HOURS/WEEK) ONE-YEAR APPOINTMENT WITH THE OPPORTUNITY FOR EXTENSION DEPENDING ON PERFORMANCE.
Bachelor s degree or equivalent with more than 2 years of relevant work experience. Must be well organized and detail oriented. Demonstrated ability to work up and manage multiple projects simultaneously, balancing priorities to achieve successful outcomes. Ability to keep a comprehensive, up-to-date, and accurate laboratory notebook. Basic computer knowledge, including Word, Excel, PowerPoint, and email, is expected. Experience in and/or willingness to make, update, and maintain online databases. Experience in and/or willingness to train or supervise students or visitors. Ability to work independently as part of a research team. Ability to develop effective working relationships with faculty, staff, and students. Strong written and oral communication skills. Personable, enthusiastic, self-motivated, dedicated individual with a positive can do attitude. Ability to lift 20-50 pounds. Close concentration and manual dexterity are required.
Bachelor s degree and 4+ years of laboratory experience or the equivalent combination of education and experience. Plant molecular biology, plant biotechnology, or plant genetics education desirable. Experience with wet lab plant research. Experience with tissue culture and/or plant transformation is highly desired. Experience developing or perfecting laboratory protocols is advantageous. Previous work experience in a service facility is also a plus.
VISA SPONSORSHIP IS NOT AVAILABLE FOR THIS POSITION.
RELOCATION ASSISTANCE WILL NOT BE PROVIDED FOR THIS POSITION.
UNIVERSITY JOB TITLE:Technician IV
PAY RATE TYPE:Hourly
CONTACT NAME:Kate Nicholson
NUMBER OF OPENINGS:1
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